How to find out if two sets of data are equally at 95 % Confidence Level in R

I am interesting to find a way to identify if two sets of data can be consider statiscally different at 95 % Confidence level (or any other). To be more specific, my data sets are composed by 5 values. They corresponding to 5 readings of the same detector by one microscope. So, two detectors with 5 readings each can be represent the same value or not. The problem is to find a method to answer this questions. I'm wondering if a t-test is the proper tool. Am I right ? I would like to do the statistical test in R.

Thanks in advance