Microsoft Bot working in emulator but not in azure web chat

I have a bot developed in Bot Framework SDK 4.0, .Net Core 2.1 . I have deployed this in local IIS and when tested with emulator locally it works well. I have configured it in Azure, set message end point as below:

when I tried to test with Test "Test in Web chat" in Azure I can see

and 404 not found in ngrok in IIS server(local). I have configured Microsoft App Id & Password in appsettings.json same as Azure. Is there any thing which I am missing here? Thanks for Help.